Prime Performance
Controlled Porosity Glass (CPG)

A Unique Material ...

Controlled porosity glass (CPG) is a finely divided, micro-porous glass powder with many highly useful properties. Composed of  nearly pure silica, CPG is very chemically inert and has an extremely rigid structure. Its very high specific surface area provides many sites for chemical attachment or adsorption.  A precisely controlled, uniform pore structure enables good spatial distribution of ligands and excellent solution flow characteristics.

Since its development by W. Haller, CPG has become a valuable aid in the areas of purification, separation, synthesis, and chemical analysis.

For Many Applications

CPG is available as an uncoated glass with a wide range of porosities and particle sizes. It is also available with a diverse selection of ligands covalently bonded to its surface. Thus, it can be custom tailored for many applications including:

	Solid Phase Synthesis
	Biomolecular Sequencing
	Affinity Chromatography
	Permeation Chromatography
	Ion Exchange Chromatography
	Immunosorbent
	Extractions/Concentrations
	Immobilized Enzymes
	Chemical/Biochemical Sensors

DNA/RNA Synthesis

CPG is the solid support of choice for the automated synthesis of DNA and RNA, and Prime Performance is the CPG of choice for high quality synthesis. It offers complete freedom from swelling in all synthesis solvents, while providing a well defined pore structure for the growing oligomer.

Prime Performance CPG provides the above advantages plus an extra measure of precision in its key specifications. Advanced production techniques allow tighter control of particle size and shape, pore size, pore volume, and  specific surface area. These parameters influence solution exchange behavior, ligand loading and dispersion, and reaction kinetics, which in turn impact the efficiency, purity, and reproducibility of syntheses.

Prime Performance CPG was developed to meet the demanding needs of the anti-sense DNA research community. In order to satisfy the requirements for several different types of  large scale synthesis techniques, several sets of custom specifications were developed.  For example, a special grade of Nucleoside Prime Performance CPG has loadings of  90-110 micromoles per gram and a higher bulk density to increase the yield and lower the cost of the anti- sense product.  Product and performance documentation has been developed in conjunction with several anti-sense DNA companies to meet both their internal Q.A. and regulatory needs.

Prime Performance CPG is sold as an underivitized or Native solid support, or in a wide range of chemically modified forms including aminopropyl, long chain amine, and nucleoside supports.  It is available in all popular types of DNA synthesis columns and in bulk form.  Specifications can be custom tailored to help you optimize your process.  Extensive technical support is available to discuss your needs and specification options.

Chromatography

Prime Performance CPG provides many advantages as a chromatographic packing material including:

	Pure Fractions-High Recoveries
	High, Linear Flow Rates
	No Shrinkage or Swelling
	Aqueous and Organic Systems
	Biologically Inert Quartz Glass
	Sterilizable
	No Pretreatment Required
	Long Column Life

 

Hundreds of papers have been published on the use of CPG for various chromatographic applications. Its rigidity and insolubility provides a packing which is insensitive to changes in mobile phase composition and predictable with system pressure. Its narrow pore size distribution results in sharp exclusion limits, high resolution and excellent reproducibility. Since it does not compress,  and pores are interconnected, CPG  permits high flow rates which are linear with pressure. Prime Performance CPG enhances all of the above advantages due to its sharper pore size distribution and more tightly controlled physical and chemical characteristics.

Affinity chromatography is an adsorption chromatography on a matrix carrying biospecific ligands. Hormones, enzymes, enzyme inhibitors, enzyme substrates, coenzymes, antigens, antibiotics, etc. have been immobilized on CPG and used for isolating substances showing specific interactions with these ligands. Some of the more popular substances isolated by CPG include interferons, viruses and myoglobin.

Permeation Chromatography , also known as gel, or exclusion chromatography, separates components on the basis of molecular size. Thus, pore size and distribution are critical parameters of the packing material. While many organic polymers have been used for packings, most are very soft and deform under hydrostatic pressure and high flow rate conditions, thus changing their pore size and geometry, and retention characteristics.

The rigid nature of CPG provides for pressure independent performance and higher flow rates. This means cleaner separations, which are many times faster than with other packings. Because of these advantages, CPG has been used to isolate or purify hundreds of substances including viruses, peptides, nucleic acids, proteins, and cell components.

Surface Derivatized CPG

Through the use of silane chemistry, many different types of functionalized CPG. s can be prepared. Hundreds of different derivitized supports have been made including many organic functional groups, drugs, hormones, enzymes, dyes, antigens, and antibodies. Since CPG can have a large pore size while retaining structural strength, many forms of analytical and preparative processes can be done on large molecules, where the substance of interest can access the derivitized surfaces on the interior of pores.

A wealth of technical literature has been published on the use of derivitized CPG for affinity chromatography. Examples include ion exchange, hydrophobic, chelating and many other types of affinity phenomena.

Limitless Possibilities

The list of novel applications for CPG goes on and on. From catalytic substrates, to fiber optics sensors, to immobilized chemical reagents, to trace metal preconcentrators, CPG has shown to be a valuable tool for both research and process engineering.

Ordering Information

For custom applications virtually any combination of particle size, pore size,  and chemistry can be accomodated. Contact our technical service group to discuss your application.